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a price with the ionization of your guanidino team is predicted to exceed 12. Even so, in NagA from E. coli

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The next ionization noticed while in the log kcat/Km profile could originate from other residues within the active website of NagA but it could also arise within the protonation of the substrate alone Considering that the phosphate moiety provides a p

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It may be argued, nonetheless, that if NagA had been limited by products release from the Energetic website, then the dissociation of trifluoroacetate may very well be inherently more rapidly than the release of acetate. This certain circumstance was addressed daftar naga26 with the utilization of changes in solvent viscosity to systematically alter the rate constants for that Affiliation and dissociation of goods and substrates with NagA (19, 20).

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